: Select the specific run or order. Look for a link labeled "Download All Results" or "Download .zip" . This package usually includes the .ab1 trace files, .seq text files (FASTA format), and sometimes a .pdf report. 2. Opening and Viewing the Data
An (also known as a trace file) is the standard output format for Sanger DNA sequencing, containing the raw data, base calls, and the chromatogram (the visual "peaks" of the sequence). Scientists typically download these in a compressed ZIP archive when receiving batch results from a sequencing facility. 1. How to Download Your AB1 ZIP Download AB1 zip
: Services like the EditCo ICE tool allow you to drag and drop your .zip archive directly for CRISPR knockout or knock-in analysis. 3. Key Analysis Tips : Select the specific run or order
Inference of CRISPR Edits (ICE) - Knockout Analysis - EditCo Bio such as Eurofins Genomics or GENEWIZ
: If you are a bioinformatician, tools like the sangeranalyseR package in R can process an entire directory of AB1 files from your unzipped archive at once.
Most sequencing providers, such as Eurofins Genomics or GENEWIZ , deliver results through a secure online portal.
: Always store the original .ab1 files. While .seq files are easier to copy/paste, the .ab1 file is essential for verifying any ambiguous base calls.